ABSTRACT
Brucellosis is an endemic zoonosis in Syria, affecting large numbers of animals and there are an increasing number of cases in humans. The aim of this study is to investigate the in vitro efficacy of various traditional and new antibiotics against89 Brucella isolates (isolated from domestic animals) collected from different Syrian regions. Minimum inhibitory concentrations (MICs) of seventeen antibiotics were determined. Ciprofloxacin and ofloxacin were the most effective antibiotics, whereas sparfloxacin, levofloxacin, doxycycline and tetracycline had a moderate activity. In contrast, moxifloxacin and rifampicin had a low activity, while streptomycin, spiramycin and cephalosporines were ineffective. As a result, we come to the conclusion that a combination between one effective quinolone and doxycycline has a good efficacy against Brucella. Further in vivo studies are necessary to support this suggestion.
Subject(s)
Humans , Animals , Anti-Bacterial Agents/analysis , Brucella/genetics , Brucella/isolation & purification , Ciprofloxacin/analysis , Drug Resistance, Microbial , In Vitro Techniques , Quinolones/analysis , Methods , ZoonosesABSTRACT
The replication of Escherichia coli O157:H7 on the resident peritoneal macrophages of four mice strains (BALB/c, CD1, C57BL, and Swiss) has been investigated. Macrophagial bactericidal killing activity was estimated via studying their ability to internalize (gentamicin-protected) E. coli during 2, 4, 24, and 48 h assays. Host genetic background has been found to show no significant effect on the ability of resident peritoneal macrophages to kill E. coli O157:H7.
A multiplicação de Escherichia coli O157:H7 em macrófagos peritoniais residentes de quatro linhagens de camundongos (BALB/C, CD1, C57BL e Swiss) foi investigada. A capacidade bactericida dos macrófagos foi estimada através da avaliação de sua capacidade de internalizar E.coli (protegidos pela gentamicina) em ensaios de 2, 4, 24 e 48h. Observou-se que as características genéticas do hospedeiro não têm efeito significativo na capacidade dos macrófagos peritoniais eliminarem E. coli O157:H7.